Methods for analysis of Epimedium extract powder

Properties: icariin extract 90 brown powder

Source: This product is selected from the dry above-ground part of Herba Epimedii, a wild Berberine plant in the Qinba Mountains

CAS number: 489-32-7

Molecular formula: C33H40O15

Molecular weight: 676.66

Melting point: 231℃-232℃

Detection method: HPLC

Analytical method

1. Thin-layer identification method （98 icariin powder）

Take 0.5g of the herb powder or an appropriate amount of the extract, add 10ml of ethanol, soak it in warm water for 30 minutes, filter it, steam the filtrate, and add 1ml of ethanol to dissolve the residue, which is the test solution. Weigh the icariin control product, dissolve it with methanol and dilute it into 0.1mg/ml, that is, the control product solution. The test solution and the control solution were absorbed 10μl each on the silica gel H thin layer plate with the same sodium carboxymethyl cellulose as binder, and the solution was divided into ethyl acetate, butyl ketone, formic acid and water (10:1:1: 1) For the developing agent, unfold, remove, dry, put ultraviolet lamp (365nm) to examine, the test product chromatogram, and the control product chromatogram in the corresponding position of the same dark red spots; Spray aluminum trichloride solution, and then ultraviolet lamp (365nm) to examine, the same orange-red fluorescence spots.

2. The content of total flavonoids in Epimedium and its extracts was determined by spectrophotometry

The control product icariin was accurately weighed and the standard solution of 10ug/ml was prepared with methanol. Accurately weigh the appropriate amount of this product powder (equivalent to about 1mg containing icariin), use 25ml methanol ultrasonic chemical book for 30 minutes, fixed volume in 50ml measuring bottle, with 0.45μm microporous filter membrane filtration, that is, the test product solution. Take the test solution and the control solution, take the reagent as blank, and measure the absorption at 270nm wavelength.

3. The content of icariin in Epimedium and its extracts was determined by HPLC

Chromatographic conditions and system suitability test: ODS25uC18(4mm×150mm) column; Mobile phase: acetonitrile-water (30:70); Detection wavelength: 270nm; Flow rate: 0.8ml/min. The theoretical plate number is not less than 1500 icariin. Preparation of reference solution: The reference product icariin was precisely weighed and prepared into 0.1mg/ml solution with methanol. Preparation of test product solution: Accurately weigh the appropriate amount of this product powder (equivalent to about 1mg containing icariin), ultrasonic with 25ml methanol for 30 minutes, fixed volume in 50ml measuring bottle, with 0.45μm microporous filter membrane filtration, that is, the test product solution. Determination method: The sample solution and the control solution were injected 10μl into the HPLC instrument respectively, and the area was calculated by external standard method.

Pharmacological action

1. Effect on sexual function The extract of Epimedium has a certain role in promoting gonadal function. Flavonoids such as icariin have the function of tonifying kidney and aphrodisiac. 30 ~ 1000μg/l can promote the secretion of estradiol by follicular granulocytes, and 1000μg/l can promote the secretion of corticosterone by adrenal cortical cells. At the same time, EPS can increase the level of gonadal hormone, mainly increase the level of FSH.

2. Improving the immune function of Epimedium by 50% methanol extract can inhibit lymphocyte transformation. The total flavone of Epimedium epimedium powder can make the “Yang deficiency” mice form higher cell function and antibody, and obviously enhance the human T cell immune function. Both EPS and EI have immunomodulatory effects.

3. Antioxidant EPS and EI both have antioxidant activities, which can enhance the activity of antioxidant enzymes and reduce the effect of free radical products.

4. Anti-aging effect Epimedium extract can resist aging and prolong life by affecting cell passage, prolonging growth period, regulating immune and secretory systems, improving body metabolism and organ functions.

5. The non-amino acid part of Chemicalbook in epimedium alcohol extract can significantly increase the coronary artery flow of isolated rabbit hearts. Decoctions and alcohols can lower blood pressure. Icariin can directly relax vascular smooth muscle and dilate coronary artery, femoral artery and cerebral artery. The mechanism of action is to inhibit the intracellular calcium ion flow in vascular smooth muscle.

6. Anti-inflammatory and anti-allergic effects of Herba epimedium brevicornum methanol extract can significantly reduce the swelling degree of rat egg white “arthritis” and reduce the increase of capillary permeability caused by histamine in rabbits. It can also inhibit allergic asthma induced by histamine and acetylcholine in guinea pigs.

7. Effect on bone growth The extract of epimedium sagittatum extract can inhibit the activity of osteoclasts, promote the function of osteoblasts, increase the formation of calcified bone, and promote the synthesis of DNA of bone marrow cells, which can not only prevent osteoporosis caused by castration rats, but also prevent bone mass loss and osteoporosis caused by hormones. Other studies have shown that Epimedium injection can significantly promote the growth of chicken embryo femur in vitro.

8. Other effects The crude extract of Epimedium has expectorant, antitussive and panting effects.

Andy

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